Discover our range of Chromogenic Substrates


Chromogenic substrates are colourless soluble molecules made up of two parts:

  1. a chromophore – a chemical group that, after enzymatic cleavage, releases colour and,
  2. a specific enzymatic substrate, usually a digestible carbohydrate

These compounds are useful for enzymatic detection, as chromogenic substrates specifically bind with the target enzyme. This reaction allows the enzyme to catalyze the separation of the chromophore group, resulting in an insoluble product with a distinctive colour that confirms the existence and activity of the enzyme under study. The intensity of the colour can be quantified in chromogenic assays in techniques such as Western blot, ELISA, immunohistochemistry, enzymatic assays, and microbial detection in culture media.

Why use Chromogenic Substrates?

Chromogenic substrates have applications in various areas:

  • Microbiology: For use in culture media, diagnostic tests in clinical laboratories, identification of microorganisms, and in the food industry.
  • Biotechnology: For colourimetric detection, providing a cost-effective detection technique, from immunohistochemistry to Western blot and ELISA.

Chromogenic Substrates Overview

Our new range of Chromogenic Substrates

At DC Fine Chemicals, we have a new range of chromogenic substrates. We have sorted them according to the colour they produce, the name of the substrate, other synonyms they are known by, their CAS number, and the type of enzyme they specifically bind to.

  • Lapis Substrates (Deep Blue)
  • X-Substrates (Blue Green)
  • Magenta Substrates (Magenta to Lilac)
  • Salmon Substrates (Pink)
  • Other Chromogenic Substrates


Clasification DCFC Code Substrates Synonims CAS Enzyme
Lapis Substrates

(Deep Blue)

125290 5-Bromo-3-indolyl phosphate disodium salt Blue-phos 16036-59-2 Alkaline phosphatase

(Blue Green)


125030 5-Bromo-4-chloro-3-indolyl-β-D-cellobioside X-Cellobioside 177966-52-8 β-Cellobiosidase
125000 5-Bromo-4-chloro-3-indolyl caprylate X-Caprylate 129541-42-0 Esterase
125220 5-Bromo-4-chloro-3-indolyl-α-D-galactopyranoside X-α-Gal, X-α-D-Galactoside 107021-38-5 α-Galactosidase
124980 5-Bromo-4-chloro-3-indolyl-α-D-glucopyranoside X-α-Glucoside 108789-36-2 α-Glucosidase
124960 5-Bromo-4-chloro-3-indolyl-N-acetyl-β-D-glucosaminide X-N-Acetyl-β-D-glucosaminide; X-Glucosaminide 4264-82-8 N-Acetyl-β-D-glucosaminidase
Magenta Substrates (Magenta to Lilac)


125020 5-Bromo-6-chloro-3-indolyl-β-D-glucopyranoside Magenta-glucoside 93863-89-9  β-Glucosidase
125010 5-Bromo-6-chloro-3-indolyl-α-D-glucopyranoside Magenta-α-D-glucoside 878495-64-8 α-Glucosidase
124990 5-Bromo-6-chloro-3-indolyl phosphate disodium salt Magenta-phos 404366-59-2 Alkaline phosphatase
124970 5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside Magenta-gal 93863-88-8 β-Galactosidase
125240 5-Bromo-6-chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt Magenta-GlcA CHA salt, Magenta-gluc CHA salt 144110-43-0 β-Glucuronidase
Salmon Substrates (Pink)


125260 6-Chloro-3-indolyl-α-D-galactopyranoside Salmon-α-gal 198402-61-8 α-Galactosidase
103380 6-Chloro-3-indolyl-β-D-galactopyranoside Salmon-gal 138182-21-5 β-Galactosidase
125250 6-Chloro-3-indolyl-β-D-glucopyranoside Salmon-glucoside 159954-28-6 β-Glucosidase
125230 6-Chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt Salmon-glcA CHA salt, Salmon-gluc CHA salt 138182-20-4 β-Glucuronidase
Other Chromogenic Substrates

(Blackish purple)

125210 5-Bromo-4-chloro-3-indolyl phosphate disodium salt X-Phosphate disodium salt, BCIP 102185-33-1 Alkaline phosphatase, often in conjunction with NBT
125270 Nitro blue tetrazolium NBT, Nitro BT 298-83-9 Alkaline phosphatase


If you want to learn more about these substrates and how they can enhance your operations, we are here to help. Please contact us at to arrange a meeting with one of our team.


Article written by Sherry Cacay, Marketing Manager of DC Fine Chemicals


Discover our Chromogenic Substrates