Discover our range of Chromogenic Substrates
Chromogenic substrates are colourless soluble molecules made up of two parts:
- a chromophore – a chemical group that, after enzymatic cleavage, releases colour and,
- a specific enzymatic substrate, usually a digestible carbohydrate
These compounds are useful for enzymatic detection, as chromogenic substrates specifically bind with the target enzyme. This reaction allows the enzyme to catalyze the separation of the chromophore group, resulting in an insoluble product with a distinctive colour that confirms the existence and activity of the enzyme under study. The intensity of the colour can be quantified in chromogenic assays in techniques such as Western blot, ELISA, immunohistochemistry, enzymatic assays, and microbial detection in culture media.
Why use Chromogenic Substrates?
Chromogenic substrates have applications in various areas:
- Microbiology: For use in culture media, diagnostic tests in clinical laboratories, identification of microorganisms, and in the food industry.
- Biotechnology: For colourimetric detection, providing a cost-effective detection technique, from immunohistochemistry to Western blot and ELISA.
Our new range of Chromogenic Substrates
At DC Fine Chemicals, we have a new range of chromogenic substrates. We have sorted them according to the colour they produce, the name of the substrate, other synonyms they are known by, their CAS number, and the type of enzyme they specifically bind to.
- Lapis Substrates (Deep Blue)
- X-Substrates (Blue Green)
- Magenta Substrates (Magenta to Lilac)
- Salmon Substrates (Pink)
- Other Chromogenic Substrates
Clasification | DCFC Code | Substrates | Synonims | CAS | Enzyme |
Lapis Substrates
(Deep Blue) |
125290 | 5-Bromo-3-indolyl phosphate disodium salt | Blue-phos | 16036-59-2 | Alkaline phosphatase |
X-Substrates
(Blue Green)
|
125030 | 5-Bromo-4-chloro-3-indolyl-β-D-cellobioside | X-Cellobioside | 177966-52-8 | β-Cellobiosidase |
125000 | 5-Bromo-4-chloro-3-indolyl caprylate | X-Caprylate | 129541-42-0 | Esterase | |
125220 | 5-Bromo-4-chloro-3-indolyl-α-D-galactopyranoside | X-α-Gal, X-α-D-Galactoside | 107021-38-5 | α-Galactosidase | |
124980 | 5-Bromo-4-chloro-3-indolyl-α-D-glucopyranoside | X-α-Glucoside | 108789-36-2 | α-Glucosidase | |
124960 | 5-Bromo-4-chloro-3-indolyl-N-acetyl-β-D-glucosaminide | X-N-Acetyl-β-D-glucosaminide; X-Glucosaminide | 4264-82-8 | N-Acetyl-β-D-glucosaminidase | |
Magenta Substrates (Magenta to Lilac)
|
125020 | 5-Bromo-6-chloro-3-indolyl-β-D-glucopyranoside | Magenta-glucoside | 93863-89-9 | β-Glucosidase |
125010 | 5-Bromo-6-chloro-3-indolyl-α-D-glucopyranoside | Magenta-α-D-glucoside | 878495-64-8 | α-Glucosidase | |
124990 | 5-Bromo-6-chloro-3-indolyl phosphate disodium salt | Magenta-phos | 404366-59-2 | Alkaline phosphatase | |
124970 | 5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside | Magenta-gal | 93863-88-8 | β-Galactosidase | |
125240 | 5-Bromo-6-chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt | Magenta-GlcA CHA salt, Magenta-gluc CHA salt | 144110-43-0 | β-Glucuronidase | |
Salmon Substrates (Pink)
|
125260 | 6-Chloro-3-indolyl-α-D-galactopyranoside | Salmon-α-gal | 198402-61-8 | α-Galactosidase |
103380 | 6-Chloro-3-indolyl-β-D-galactopyranoside | Salmon-gal | 138182-21-5 | β-Galactosidase | |
125250 | 6-Chloro-3-indolyl-β-D-glucopyranoside | Salmon-glucoside | 159954-28-6 | β-Glucosidase | |
125230 | 6-Chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt | Salmon-glcA CHA salt, Salmon-gluc CHA salt | 138182-20-4 | β-Glucuronidase | |
Other Chromogenic Substrates
(Blackish purple) |
125210 | 5-Bromo-4-chloro-3-indolyl phosphate disodium salt | X-Phosphate disodium salt, BCIP | 102185-33-1 | Alkaline phosphatase, often in conjunction with NBT |
125270 | Nitro blue tetrazolium | NBT, Nitro BT | 298-83-9 | Alkaline phosphatase |
If you want to learn more about these substrates and how they can enhance your operations, we are here to help. Please contact us at info@dcfinechemicals.com to arrange a meeting with one of our team.
Article written by Sherry Cacay, Marketing Manager of DC Fine Chemicals