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Biological buffers
When we talk about successful chemical procedures, biological buffers are often implied. Indeed, their presence ensures that the biological activity of a given process leads to positive or negative experimental results.Leer más
Stable pH compositions
A biological buffer system describes a composition made up of a weak acid, which donates protons, and its conjugate base, which accepts protons. Although commonly known as biological buffers, they are also referred to as buffer solutions, buffering solutions, or simply buffers. The primary purpose of a biological buffer system is to maintain stable pH levels when dealing with a mixture containing varying amounts of strong acids or bases. It is worth mentioning that there are several types of biological buffers, each with its own pH range. Thus, each laboratory process has specific requirements, which depend on the buffer system employed. Depending on the choice, there will be advantages and disadvantages. The pH range of the compounds used in an experiment must match the pH levels of the biological buffer itself. This is an essential aspect to consider for maintaining vital processes. The main differentiation is between organic buffers (amino acids, hemoglobin buffer, etc.) and inorganic buffers (phosphate buffer, carbonate buffer). To choose the ideal biological buffer system for each procedure, the characteristics to consider include:- Temperature. It can modify the buffering capacity of the buffer.
- Reactivity. Ensuring that adverse reactions are not a possibility.
- Toxicity. It can be harmful to the test sample.
- Concentration. Higher concentration is needed the more the pH-altering element is used.